中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (51): 9611-9615.doi: 10.3969/j.issn.2095-4344.2012.51.021

• 生物材料基础实验 basic experiments of biomaterials • 上一篇    下一篇

带官能团有机磷改性羟基磷灰石的可行性

赵丽萍，周晓勇，李翠翠，钟银屏，谢兴益   

  • 出版日期:2012-12-16 发布日期:2012-12-16

Feasibility of in-situ modifying hydroxyapatite with functional organophosphorus compounds  

Zhao Li-ping, Zhou Xiao-yong, Li Cui-cui, Zhong Yin-ping, Xie Xing-yi   

  • Online:2012-12-16 Published:2012-12-16

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摘要：

背景：由于纳米羟基磷灰石粒子易团聚，由其制得的复合材料力学性能始终达不到皮质骨的水平。
目的：通过新方法制备稳定的羟基磷灰石胶体，克服粒子团聚。
方法：在0.05 mol/L硝酸钙溶液38 mL中分别加入等体积的2-氨乙基磷酸，3-膦酸基丙酸，L-O-磷酸丝氨酸溶液。将7.5mL的0.15 mol/L磷酸三铵溶液分别加入到上述每种混合液中，在40，80℃各老化4 h。所得的有机磷改性羟基磷灰石通过离心和水洗提纯，冷冻干燥得粉体。
结果与结论：红外光谱和X射线衍射证实改性后的物质仍为羟基磷灰石，表面改性剂接枝量很少。Zeta 电位分析表明3-膦酸基丙酸-羟基磷灰石、L-O-磷酸丝氨酸-羟基磷灰石可带上负电荷，2-氨乙基磷酸-羟基磷灰石可带上正电荷。3-膦酸基丙酸-羟基磷灰石、L-O-磷酸丝氨酸-羟基磷灰石的粉体在水中超声4 h，得到碱性条件下稳定性较好(3 d)的淡蓝色胶体；2-氨乙基磷酸-羟基磷灰石再分散胶体在酸性条件下的稳定性略好于中性条件，和碱性条件下相当，但都不理想(<3 h)。表明提高羟基磷灰石胶体的稳定性还需探索新的方法。

Abstract:

BACKGROUND: Nano-hydroxyapatite particles agglomerate so easily that their polymer-based nanocomposites are inferior to cortical bones in mechanical properties.
OBJECTIVE: To prepare stable hydroxyapatite (HA) colloid by new methods.
METHODS: 38 mL calcium nitrate solution (0.05 mol/L) was mixed with an isovolumetric solution of 2-aminoethyl dihydrogen phosphate (ADP), 3-phosphonopropionic acid (CEPA) and L-O-phosphoserine (LOP), respectively. Thereafter, 7.5 mL triammonium phosphate solution (0.15 mol/L) was dropped into each of the mixtures followed by aging at 40 ℃ and 80 ℃ for 4 hours, respectively. The modified HAs (ADP-HA, CEPA-HA, LOP-HA) were purified through centrifugation and water washing, followed by freeze drying.
RESULTS AND CONCLUSION: The as-synthesized products were still HA as proved by Fourier transform infrared spectroscopy and X-ray diffraction analyses, with some modifiers on surface. Zeta potentials showed that the CEPA-HA and LOP-HA were negatively charged while the ADP-HA was positively charged. The CEPA-HA and LOP-HA were re-suspended in water through 4-hour ultrasonication, forming light blue colloids with short-term (3 days) stability at pH 11. The ADP-HA suspension possessed slightly higher stability in acidic solution than that in neutral one, similar to the case in alkaline solution. But its stability in all cases (< 3 hours) was far from an ideal one. Creative methods should be under way to improve HA hydrocolloids’ stability.